FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13647383
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1134150.8310384489099485No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT887930.6506229069705158No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA759190.5562898029607581No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG697770.5112848375399152No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC662880.48571949655109703No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA632910.4637592423397219No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG617710.45262157587282487No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC583970.42789888728117326No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT559030.409624321380883No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG492860.36113883518913475No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG477260.3497080722362668No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT464320.3402264009151058No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG461760.3383505834048916No Hit
CCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCAT436090.3195411164177044No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT422940.3099055694414086No Hit
CCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACT400540.29349216622703417No Hit
GGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGG389460.2853733935656382No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG350050.25649606228534805No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG346300.25374828272937017No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC326840.23948913868688232No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT298390.21864265112219683No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG291790.2138065591036758No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG275020.20151848892934274No Hit
AGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGAT273240.20021420956677188No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA272350.19956206988548647No Hit
GCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG259510.19015367268581823No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC259360.1900437615035791No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG251760.1844749282701306No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC251150.1840279561290249No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG248350.18197628072722807No Hit
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG247130.18108233644501662No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT244650.17926513823199658No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG235210.1723480611630816No Hit
CCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGT225450.16519650690538984No Hit
CGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC224870.16477151700073192No Hit
CCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAAT218890.16038972453546588No Hit
CGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGTAGCG213120.15616180772533458No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC210000.153875655134761No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG202890.14866586509662694No Hit
CGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCG202100.14808699953683427No Hit
CCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT201940.14796976094244588No Hit
AGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGA200100.1466215171069794No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT199230.14598403224999254No Hit
CAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG198880.14572757282476795No Hit
CCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATT197930.1450314686705869No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA194170.14227636170245972No Hit
CCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTTAAAGTT193140.1415216382510845No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA188090.13782129511570096No Hit
CATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGC181730.13316106098876246No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG180140.13199600245702783No Hit
GACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGC177830.13030337025054547No Hit
CCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTC170830.12517418174605344No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG165620.12135660001628151No Hit
AGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCG164510.12054325726771206No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA163250.1196200033369035No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT158570.11619077445104309No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC158440.11609551809310253No Hit
GGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGC157530.11542872358751856No Hit
CCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCA155580.11399987821841008No Hit
TCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGA155200.11372143655673767No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG153720.11263697955864506No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT151270.11084176358207283No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT151010.11065125086619171No Hit
CATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCAC146850.10760304741209359No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT145260.10643798888035896No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGG144070.10556602683459533No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG143430.10509707245704175No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC139930.10253247820479575No Hit
CCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTA139600.10229067360386969No Hit
TACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTA138760.10167517098333065No Hit
CGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTC137620.10083984599831339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCA103750.048.672971
TAGCCCC32300.043.975561
TGCGACA13200.043.1757774
GCGACAT13550.042.761395
ACCGCCA77200.042.582531
TAGCCGC11300.042.040151
CACGAAT197250.041.558045
ACCTACG113050.040.963212
TCACGAA199700.040.7629474
TACCGAC10400.040.6536331
TAGCCCG8650.040.0912321
ATCACGA203600.040.005453
CATCACG204750.039.8992272
CCTACGG117450.039.8709683
TACGGAA118200.039.6580125
CGGAAAC127500.037.3230977
AATGGGG220900.037.2580269
ACGGAAA129400.036.224426
CACCTAC130100.035.2364961
TACCGCT23300.034.660571
Produced by FastQC (version 0.11.5)