FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22804235
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG320070.14035550852725384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA105250.042.08251614-15
TGTCGGG47100.038.4372835
CGGGGAT223350.036.054968
TCCGACC66700.035.9091079
TTCCGAC68900.034.8268788
TGGTCGT234450.034.27192314-15
TCGGGGA237000.034.1641547
CTGTCGG55500.031.6673494
GTTCCGT30800.030.606251
GTCCCGT34150.030.4416621
CCCCGTT58850.029.4920142
GCCCCGT45900.029.3667431
GGTGTGC129400.029.3639036
GTGTGCT167700.028.3286347
CCGTTTC95300.027.9636764
TCGACCG167700.027.84146726-27
GTCGGGG68450.027.6587336
ATCGGGG175750.027.1312876
CCCGTTA30450.027.0515293
ATAGCGC100000.026.68501394-95
Produced by FastQC (version 0.11.5)