FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7425413
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG98940.13324511377346956No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT90770.12224235877519539No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA76070.10244548013692975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.061.4813651
ATCAACG101600.046.8172573
CAACGCA102850.046.248265
TCAACGC105300.045.228744
GTATCAA110550.044.3325731
ACGCAGA110150.043.099247
CGCAGAG110900.042.6476978
AACGCAG116000.041.0787286
GCTCTAG18750.039.7000051
GCAGCGC21850.038.663128
GCAGAGT123850.038.1411069
GTACTTT79750.035.6181
TGCAGCG24950.032.9048427
TACTTTT94800.029.4521792
ACTTTTT95600.029.0811713
TATCAAC180950.026.6160032
CATGCAG32750.026.3578245
CAGCGCA35900.025.5208479
GAGTACG129950.025.1877812-13
ACGGGAC14700.025.1088753
Produced by FastQC (version 0.11.5)