FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23350154
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG433600.18569470676724445No Hit
GGAGTCTTGGAAGCTTGACTNCCCTACGTTCTCCTACAAATGGACCTTGA265130.1135452896798882No Hit
CCTCTGTCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG257480.11026908002405465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA236650.057.0462414-15
TGTCGGG57150.051.4948465
GGTGTGC233800.048.1594056
GTGCTCT317450.045.3173039
CTGTCGG63150.045.2069474
CGGTGTG84550.044.863495
TCGGGGA300600.042.4086577
TGTGCTC341100.042.0595178
CGGGGAT306200.041.5036248
GTGTGCT384100.038.1649257
GTCGGGG83400.037.9099586
TGGTCGT336400.037.35591514-15
CCGGTGT60900.034.8685044
ATCGGGG213800.034.0455256
TCTGTCG84150.033.2449723
TATCGGG172800.031.6906345
TCTATCG154850.031.2390233
CCCGTTA26500.030.9224173
CTATCGG165500.030.6926844
GTCCCGT31250.030.5927731
Produced by FastQC (version 0.11.5)