FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004914274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16001206
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG13702098.563160801754567No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT9199475.749235401381621No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4508592.8176563691511753No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT3974322.4837627863799767No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG3387312.1169091879699566No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1897411.1857918709377282No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG1503450.9395854287483081No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1398780.874171609314948No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA1143250.7144773962662564No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC842370.5264415694667015No Hit
CCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCACTCCGCACC833800.5210857231636166No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA671650.41974961137304273No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC566030.35374208669021573No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC520010.32498175450025457No Hit
CCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGA427860.26739234530197287No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG406160.2538308674983623No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC397510.24842502496374336No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG371690.2322887412361293No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC352020.21999591780769526No Hit
TACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG345620.21599621928497142No Hit
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT344170.21509003758841677No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG340320.2126839689458407No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG317870.19865377647159846No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGG305420.19087311293911222No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC304300.19017316569763554No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA301820.18862328252008007No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC291540.18219876676795488No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG288630.18038015384590386No Hit
CCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG286670.17915524617331968No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA271730.1698184499343362No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC269010.16811857806217856No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT258010.16124409622624694No Hit
GGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCT253640.15851305207869956No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC225630.14100812151284098No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT225270.14078313847093774No Hit
CCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCACTCCGCAC219570.1372209069741368No Hit
GACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACAT202040.12626548273923852No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG199310.12455936133813914No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGA193360.1208408916177943No Hit
ATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATT184420.11525381274386443No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT183340.11457886361815478No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG176330.11019794382998381No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA174820.10925426495977865No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA168410.10524831690811305No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG163990.10248602511585689No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG163270.10203605903205046No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC161010.1006236654912136No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)