FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22013481
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG343920.15623153830146172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC124950.057.547019
TTCCGAC127800.056.3352748
TTTCCGA149650.048.078197
GTTTCCG138250.044.3189776
GTTCCGT33700.040.9854971
GCTCCGT101000.040.6971131
CGTTTCC144600.040.4039045
CTCCGTT113850.039.4716572
CCGTTTC145400.039.4644744
TCCGTTT147000.039.228653
CCCCGTT59150.033.614582
ATAGCGC190250.033.3766294-95
GTCCCGT32700.032.8040771
CCCGTTA25850.032.498043
TCACCCC367700.032.2179079
CCGATCG208600.032.17695286-87
GTTCACC372050.031.6738687
GCCCCGT41450.031.2611731
GATCGGC215700.031.11622288-89
ACCCGAT215800.031.11484384-85
Produced by FastQC (version 0.11.5)