FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14238316
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG169600.11911520997286476No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG160580.1127801911405815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC72150.052.623289
TTCCGAC75700.050.153018
TTTCCGA87000.043.584337
GTTTCCG79750.039.335476
GTTCCGT22550.037.464561
CGGGGAT133850.037.405638
CCGTTTC86150.037.3514824
TCGGGGA136650.036.7780957
CGTTTCC85700.036.3282285
CCCCGTT42950.035.353572
TCACCCC243200.035.028799
TCCGTTT91350.033.926423
ATAGCGC139050.033.40484694-95
CTCCGTT68900.033.1951182
GCTCCGT62650.033.181821
GTTCACC258100.033.1520657
GTCCCGT22750.032.754081
CCCGTTT50450.032.3616523
TGTCGGG29150.032.2364965
TTCACCC266500.031.8934338
Produced by FastQC (version 0.11.5)