FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9117612
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG700370.7681506955988038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7600.042.0726051
AGGGGGG76800.039.5526281
ATCGGAA10400.032.4102632
GAATCGG8850.030.63560740
ACACGTC11450.028.22373413
AATCGGG9800.027.3118941
ATCGGGG8650.026.92408842
TCGGAAG12750.026.436463
CACTCCG13450.025.58052631
ACTCCGG13000.025.39667332
CCGGAGA13200.024.48732235
CGTCTGA13150.024.04680616
CGGAAGA14850.022.9319694
CGGAGAA14400.022.4478236
TCCGGAG15150.021.56454734
ACGTCTG14850.021.52809115
CTCCGGA15450.020.92040433
CACGTCT17450.018.71853314
CACACGT20150.016.21016512
CGTTGGA37200.015.6026611
Produced by FastQC (version 0.11.5)