FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7183121
Sequences flagged as poor quality0
Sequence length35-75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24850.017.3649371
AACGGGA23300.014.2573518
AAACGGG30400.014.1944957
ACGGGAT18200.013.1719389
GGAAACG31850.012.7957765
GAAACGG32550.012.7310296
CGGGATT17200.012.54397410
CGGGAAT10750.012.42450810
AGGGGGG21000.012.0681721
AACGGGT7650.011.6395178
CGTAAGC3107.3402945E-49.942965523
ACGGGTT7709.458745E-119.3401019
CGAAAGT3600.00287074398.5618791
GTTGGAA55900.08.4546642
GTGCGTT10254.1625208E-78.427772569
GCTAGAC24150.08.08315229
ACAGGCG39350.07.919899518
GATTTCG11953.6379788E-127.73793275
ACGGGAA12103.6379788E-127.64190159
TCGTTGG17650.07.5673349
Produced by FastQC (version 0.11.5)