FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11964314
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG153700.022.2185461
CGTTGGA74250.019.4623581
ACGGGAT52950.018.1836289
CGATCGG5951.03773345E-817.888636145
AACGGGA67750.017.180968
AAACGGG88600.015.9766267
GAAACGG92850.015.56203656
GATCGGC12403.8198777E-1115.259839145
GGAAACG93750.014.4924845
TGCGTCG6100.001288602113.57125145
ATCGGAC7954.623666E-513.38835145
AGCTCGG34300.012.412522145
CACGTCG7150.004245002811.578269145
CCCGTCG10705.9686485E-511.052687145
GATCGGG14651.3512959E-59.687134145
CGGAAGC36200.09.474167145
GTTGGAA171400.09.4369022
GCGCACC29901.03682396E-108.70168145
AAGCTCG20651.1351403E-68.590587145
AACGGGT23100.08.3983418
Produced by FastQC (version 0.11.5)