FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8064146
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101330.1256549670603682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3300.022.116341
ATCGGAA4800.017.3728142
AGGGGGG26650.017.3445221
ACACGAC3859.7103947E-411.43003370
TCGGAAG6107.2759576E-1211.392013
CGTTGGA25650.011.110521
AATCGGG5006.825212E-810.42659841
ATCGGGG4753.7825157E-710.24367442
GAAACGG29750.010.1609076
CGTCTGA6908.731149E-1110.07119716
AAACGGG29950.09.8608467
GAATCGG5007.634917E-79.73149140
AACGGGT7605.4569682E-119.6006478
ACACGTC7001.204171E-99.43095613
CGGAAGA9500.09.1435864
TGCGGTA3050.00634760049.1158691
AACGGGA23450.09.0381978
GGAAACG32250.08.9422865
CCCAAAG129800.08.8626011
CACTCCG7302.4521796E-88.56847831
Produced by FastQC (version 0.11.5)