FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9385339
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120100.12796554285359324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG37550.016.4726661
CGGGATT27050.014.77148710
ACGGGAT28700.014.7696179
CGTTGGA40450.014.6044171
AACGGGA39350.014.0392448
AAACGGG46950.013.6906847
GAAACGG47850.012.7798786
GGAAACG51200.012.2830035
GATCGGA3003.7008027E-511.5833051
TGCGTTC17500.011.20019270
GTGCGTT14650.08.7187569
CGTATGC5201.3151679E-58.689054546
GTTGGAA80250.08.6592632
TATGCCG4057.615705E-48.58182848
ATCGGAA4957.010435E-58.42312
AACGGGT9102.1464075E-108.3998718
CGGGAAT16750.08.297338510
CTCGTAT3800.0040958078.231647544
GCTAGAC32500.08.12591829
ACCGCGC13600.07.92123231
Produced by FastQC (version 0.11.5)