FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6209643
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG79160.1274791481571485TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA41750.087.118231
TCGGAAG42350.085.716562
CGGAAGA46950.077.469353
AGAGCAC83400.044.801527
AGCACAC85800.043.383069
GAGCACA89050.041.9589738
GAAGAGC91650.040.6139265
CGATCGG2351.2691908E-534.787052145
GCGATCG2052.3204723E-433.231533145
AAGAGCA125250.029.77536
GGAAGAG140600.026.9784264
TATGCCG29300.023.6671945-49
CGTATGC31450.022.04924245-49
CTCGTAT29500.021.87143940-44
TTACTCG31700.021.29249430-34
TACTCGA33050.020.5945135-39
ATGCCGT30150.020.1308445-49
TCGTATG35100.019.67477640-44
CGTCTGA38900.019.57504815-19
ACACGTC39050.019.2819810-14
Produced by FastQC (version 0.11.5)