FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3057962
Sequences flagged as poor quality0
Sequence length35-75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6600.055.012945
TATGCCG6500.054.8053147
TACTCGA6650.054.08418335
CTCGTAT6300.053.82663743
TTACTCG6750.052.26802434
ATCGGAA7850.051.4882161
TCGTATG7300.050.20691744
GCCGTCT6600.049.82300650
TCGGAAG8350.048.8153042
ATGCCGT7150.048.385848
CGGAAGA8300.048.2840043
TGCCGTC6900.047.65678449
CGTCTGA8250.047.3310815
ACACGTC8150.047.0712712
TCTCGTA5200.045.45151542
ACGTCTG8900.043.87431714
ATCTCGT5650.041.2252341
CACGTCT9750.040.75199513
CGTCTTC8400.040.3699852
CCGTCTT8750.039.53811351
Produced by FastQC (version 0.11.5)