FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6798565
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131130.19287893842303486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG30750.027.573341
GATCGGA1958.401474E-616.0380881
CGTTGGA20150.011.5543211
GTCCGGA2250.007085313610.8086326
TATGCCG2600.001694734310.69292448
ACCGCGC9800.010.637115531
ACGGGAT14850.010.5277339
GGAAACG24500.010.3517085
AAACGGG24650.010.2884887
GCCACCG24600.010.02835328
GAAACGG25700.09.868366
TCGGAAG4653.2003918E-69.7128273
AACGGGA20050.09.7033678
CACCGCG10850.09.60750530
CCGGCTA14900.09.5625638
CGGCTAA15650.09.32634739
GCGTGAG26600.09.27331722
GTCACGA4552.605597E-59.1638829
CCCAAAG104150.09.1085251
AACGGGT6553.9000952E-89.0168838
Produced by FastQC (version 0.11.5)