FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7332320
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT656830.8958010561459402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338170.4612046391865058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228850.3121113099264626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197260.26902808388068167No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG138670.1891215877103018No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC130610.17812915966569925No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC113770.15516234970650492No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA105570.14397898618718222No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG97690.13323204661007704No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG88700.12097126148340497No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91200.087.627771
GCGTACT3400.082.258271
GCGTAAG4750.071.407171
GTATCAA268700.068.0099951
CGTACTT4350.061.557912
GCAGCGC15700.057.6042028
ATCAACG321900.056.6587683
CAACGCA326450.056.160725
TCAACGC328600.055.8113754
GCTCTAG12050.055.8022841
TGCAGCG16850.053.3196537
AACGCAG345000.053.153966
ACGCAGA372000.049.21477
CGCAGAG374850.048.840518
GCGTAAC1251.2732926E-1147.6047861
TGGTATC28450.047.270132
TATCAAC392050.046.3692782
GCAGAGT400000.045.7399189
GTGGTAT30500.045.2635651
GCGTACC1508.54925E-1139.670651
Produced by FastQC (version 0.11.5)