FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13951958
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1285680.9215050676041312No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC809560.580248306366748No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG726660.5208301229117805No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG390130.2796238348767965No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG386470.2770005471633444No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC361440.2590604128825503No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC358760.2571395355404596No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG318870.22854856644493912No Hit
GGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCC312700.2241262480864693No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT306960.22001213019706625No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGG258910.18557251964204594No Hit
CGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAAGCCA250810.1797668828991601No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT246680.17680672490556523No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT221650.1588665906247711No Hit
TATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT210980.15121891852025357No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT209870.15042333126289514No Hit
CCCAAGGATAAGTCATTGGCATTCGGAGTTTGACTGAATTCGGTAACCCG205480.1472768194973064No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTA176560.12654854608937327No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC175250.12560960977663493No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG168880.12104394236278519No Hit
GGGTTGTTTCCCTCTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCC161990.11610556740494775No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC156080.11186960281847178No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA155890.11173342121586088No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAA155700.11159723961325No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC154750.11091633160019547No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTG149960.10748312172384693No Hit
ACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCC148800.10665169720264353No Hit
ATCAACCTGATCATCTTTCAGGGATCTTACTTCCTTGCGGAATGGGAAAT146340.10488850382147079No Hit
CCTTGCCGAAACAGTGCTCTACCCCCGGAGATGAATTCACGAGGCGCTAC142500.10213620195817677No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT140260.1005306925379219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG106250.069.205265
CAGACGT110000.066.630096
AGACGTG112300.065.3507467
TGCGACA28250.062.3792534
GCGACAT30250.058.41375
CTGCGAC31750.056.1008073
GTATAGG146800.053.1286353
AGATCAG140400.052.3705332
GGGGCGA149650.052.3416378
GCTGCGA34600.052.166372
GATCAGA146050.050.409423
ATAGGGG156900.050.0434275
GACGTGG148050.049.634698
GGTATAG158250.049.224722
TATAGGG160000.048.7161874
GTAGCAA290150.048.326959
GGGTATA163550.048.277671
GTAGTAG291450.047.8181956
ACGTGGC153550.047.7322279
AGGGGCG165300.047.4434247
Produced by FastQC (version 0.11.5)