FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15853690
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1499640.9459248919336759No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT682810.4306946836982431No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT588310.3710871096886592No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG580430.36611665801463256No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC479590.30251001501858554No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC459010.289528810012054No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA444200.28018713624399116No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA416850.2629356320200534No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC369970.23336522916746824No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG327170.20636835966894776No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT264760.16700213010346487No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG251220.1584615316686525No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA238230.15026785562225578No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC228530.14414940622656303No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG226980.14317171585921007No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT215400.13586742266311502No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT184130.11614330796174267No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT182880.11535484798807091No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA178770.11276239159463822No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG175890.11094577981529852No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG175340.11059885742688295No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG171590.10823347750586773No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT171320.10806317015155463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT27200.055.0109185
TGCGACA28750.052.0411724
CTGCGAC33400.045.3658943
CGACTCC166250.042.3911676
CCGACTC171550.041.146735
GTAGCAA123400.040.484619
TATTCGT115000.038.7014352
TAGTAGC131250.038.0282677
ACTCCCT186000.037.9395838
TAGAGCC135550.036.9270137
CTCACGT410800.036.5065779
AGTAGCA137700.036.3490758
CCCGACT200550.035.7151954
TTCGTAC125550.035.448454
ATTCGTA125500.035.4256173
GCTCACG423950.035.4076048
GACTCCC199650.035.2994277
ACCCGAC199850.035.247013
TTAGAGC143550.034.9021646
AACCCGA203850.034.8116952
Produced by FastQC (version 0.11.5)