FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27314391
Sequences flagged as poor quality0
Sequence length41
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTC853590.31250559457833055No Hit
GCGCCGAAAATGTACCGGGGCTAAACGTATCACCGAAGCTG501320.18353694944177962No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCG420530.15395913458220614No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTG413440.15136343329053173No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTT394060.14426827235503806No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG368990.13508996045344743No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGG350500.12832063508207084No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG340910.12480966535186526No Hit
GTAGTCGAAAGGGAAACAGCCCAGACCGCCAGCTAAGGTCC338470.12391636335585882No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTC301020.1102056421466618No Hit
ACCTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACC294580.10784791064900551No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAG283660.10385001810950133No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAA276330.10116645104772792No Hit
CGTGCAAGGTTAAGCAGAAGATGCGGAGCCGCAGCGAAAGC275520.10086990407364381No Hit
GGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATC274910.10064657857464221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC76300.028.73721319
CGTACAG76650.028.54085721
ATTCGGG115400.027.90115213
GTAACGC27000.027.67750223
GAACCCG118550.027.1799925
CGTTTCG71300.027.02865233
CTACGCA34100.026.8269941
TACGCAT37450.026.299912
TACCGAC18350.026.1148666
CTACAAC84100.026.08026534
TACCCGT20700.025.9910551
GGATTCG124300.025.8609811
GCGCCAT97000.025.5911735
ACCTCGT56100.025.4907841
AGATCGT76250.025.45820829
ACCCGAC128350.025.41987827
TGAACCC128050.025.3138724
ATATCGA45400.025.2563835
TCGTTCC68800.025.0443384
CTCCGTA87600.024.991918
Produced by FastQC (version 0.11.5)