FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004914409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40298425
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA2970580.7371454343438981No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2767250.6866893681328737No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1999660.4962129413246299No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1543590.38303978381289094No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1396240.34647507936104205No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG1290240.3201713218320567No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1163170.28863907212254575No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC1032670.25625567252318177No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC1018510.2527418875551588No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC981080.2434536833635558No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG968900.24043123273428177No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC897520.22271838167372543No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG892990.22159426826234524No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG889760.220792748103679No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG817090.20275978527696803No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC748930.1858459728885186No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG727040.1804139988101272No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA699760.1736445034762525No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG680240.16880064171242423No Hit
GGTACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC660120.1638078907550357No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA659550.16366644602115343No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA653850.16225199868233064No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG641090.15908562183261504No Hit
GGCATGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC633890.15729895150989154No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG623630.1547529463000105No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG601900.1493606760065685No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA591780.1468494116085182No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA589040.1461694842912595No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA560580.13910717354338292No Hit
GGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTG548780.13617901940336377No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA546540.13562316641407202No Hit
GGCGCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC536350.13309453161010634No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG532070.13203245536270958No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA529070.13128800939490812No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA528610.13117386101317857No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG524250.1300919328733071No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC491960.12207921277320392No Hit
GCGCCGAAAATGTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTT482220.11966224486440848No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGC468910.11635938625392928No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG465430.11549582893127958No Hit
GGCCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG461680.11456527147152773No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT458590.11379849212469222No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA449220.1114733392185923No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT436170.1082349992586559No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG432010.10720270084997119No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG423250.1050289186239909No Hit
GTCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA422250.10478076996805706No Hit
GGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAAT414670.10289980315607869No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC411300.1020635421855817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)