FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004914424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170204
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCNGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT422150.4603496279908277No Hit
AAACCNAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG381290.4157922768130349No Hit
CCCCGNGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG322440.35161704145294914No Hit
CTCAGNACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT276620.301650868399438No Hit
CGCCCNATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC269440.29382116253902313No Hit
CACGANGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC263790.2876599037491423No Hit
AACTGNTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG182990.19954845061244003No Hit
AGCCCNTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC178170.19429229709611695No Hit
GTATTNGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA158510.17285329748389458No Hit
CTCCCNGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT139560.15218854455146252No Hit
TGGCTNTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA112010.12214559239903497No Hit
CCGCCNCTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG110780.12080429181291932No Hit
GCACGNCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC104700.11417412306203875No Hit
AAACCNAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA92450.10081564161495207No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)