FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004914426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9645008
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCNGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT443050.4593567988746095No Hit
AAACCNAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG402050.41684776207546953No Hit
CCCCGNGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG323310.33520967530560886No Hit
CTCAGNACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT287420.2979987160197275No Hit
CGCCCNATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC283070.2934886108959163No Hit
CACGANGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC278110.28834605424899595No Hit
AACTGNTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG188200.19512684696580865No Hit
AGCCCNTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC180870.18752706063074287No Hit
GTATTNGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA161190.16712272296715563No Hit
CTCCCNGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT139220.14434410007747012No Hit
CCGCCNCTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG117300.12161731747656404No Hit
TGGCTNTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA117080.1213892202059345No Hit
GCACGNCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC107560.11151882922232931No Hit
AAACCNAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA99200.1028511329384071No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)