FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004914430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9546524
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCNGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT433650.454249106795311No Hit
AAACCNAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG397900.4168009214662845No Hit
CCCCGNGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG319440.33461393906305587No Hit
CTCAGNACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT288700.3024137371885306No Hit
CGCCCNATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC278050.29125784421638706No Hit
CACGANGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC272490.2854337348337468No Hit
AACTGNTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG186190.19503433920032043No Hit
AGCCCNTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC178750.1872409266451328No Hit
GTATTNGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA161390.16905629734969502No Hit
CTCCCNGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT139900.14654548608477808No Hit
TGGCTNTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA118050.12365757421235206No Hit
CCGCCNCTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG113680.11907999183786684No Hit
GCACGNCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC103910.10884590035074547No Hit
AAACCNAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA95700.10024591149616342No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)