FastQCFastQC Report
Thu 12 May 2022
EGAF00004914432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30871001
Sequences flagged as poor quality0
Sequence length101
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG5143741.6662044745487845No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4011011.2992808364069568No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC3098701.0037575393165905No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG2792610.904606235476459No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2211950.7165138571308394No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1421210.4603705594126993No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA1311040.4246833460307944No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG1300840.4213792743552436No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1268290.4108353985670889No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1251720.4054679017372971No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1244150.40301576226828534No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC1201880.38932330053048814No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1200870.3889961326488895No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC1097150.355398258708877No Hit
ACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCG1065740.3452236615197544No Hit
TACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCC1048310.33957758609771027No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC1046010.33883255032773313No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG971670.31475169852768947No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA969050.3139030056071068No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC849570.2752000169997727No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG822660.2664830984910402No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG781950.25329596536244486No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT752560.2437757039365196No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT752210.2436623289280448No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG685610.222088684458272No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT668030.21639401974688158No Hit
ACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCA641820.2079038512550986No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG629470.203903333098917TruSeq Adapter, Index 2 (100% over 49bp)
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG617450.20000971137929735No Hit
ATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGG613930.1988694827226367No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC601740.19492079314175786No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCA587710.1903760749448973No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC582540.18870136410542696No Hit
ACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGC577730.18714326756038782No Hit
ACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCC575610.1864565389376263No Hit
GTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCG571680.1851834995567523No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA570780.1848919638206743No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG566380.18346667799984845No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG562990.1823685600606213No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC551790.17874055978942827No Hit
CACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGG530880.17196721285454916No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA521230.1688413019066016No Hit
CCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGC499050.16165656565525685No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT498210.16138446563491737No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG497790.16124841562474762No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA482720.15636681168841918No Hit
CCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGC480420.15562177591844203No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT473200.1532830114579051No Hit
GCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGA467460.15142366131891868No Hit
GACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCG466950.15125845773514116No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC466010.15095396485523743No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG454980.14738103244530362No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG444990.1441449857748377No Hit
TACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACG444970.14413850720292484No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT443290.1435943071622459No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC432160.13998898189274783No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG425970.1379838638857224No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG425370.13778950672833706No Hit
TAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAG424030.13735544241017647No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG423420.1371578459668347No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA420500.13621197446755937No Hit
AATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCT418040.13541511012227947No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC416830.1350231565215524No Hit
TACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACC415290.13452430648426333No Hit
TACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGC411810.13339703497142835No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA405370.13131093481549239No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG402910.1305140704702125No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG401470.13004761329248768No Hit
CAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACG397300.12869683104865956No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA386610.12523403436124406No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT379980.12308638777213607No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG377850.12239641986341811No Hit
ACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGA376120.12183602339295703No Hit
TACCCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCC373200.12089015189368171No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC363680.11780635166316765No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA360490.11677301944306893No Hit
GGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGG352150.1140714549554127No Hit
GCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT346190.11214084052538498No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG339450.10995756179075632No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG331170.10727543301883861TruSeq Adapter, Index 1 (100% over 49bp)
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC325430.1054160828798522No Hit
CCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCC325170.10533186144498523No Hit
CCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCAT317710.10291535412149415No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGAC316370.10248128980333356No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA316320.10246509337355145No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC314700.10194032904861103No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT312910.10136049686241143No Hit
CAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCC312690.10128923257137012No Hit
TACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCT309520.10026237892318424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)