FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7732576
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG188990.24440755577442758No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC122310.1581749729973556No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC115200.14898010701737688No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA114260.14776447072747814No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG103030.13324149675347516No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG101840.1317025529396672No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT90130.11655882851975849No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG85130.11009267804157373No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC84750.10960125060523168No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC83820.10839854661628932No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG83500.1079847129856855No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG83440.10790711917994728No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG81250.10507494527050236No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA80780.10446712712555298No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG80430.10401449659208006No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT80190.10370412136912717No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG77870.10070382754724945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG12900.069.71311
GGTATCA20400.061.5998151
GCAGCGC18400.056.253118
TGCAGCG18400.055.9276437
CAACGCA60900.051.507785
CGCAGAG63650.048.972028
ACGCAGA64350.048.529867
TGGTCCT29250.045.9898575
ATCAACG69050.045.2558943
TCAACGC70350.044.5882454
CATGCAG23450.044.1657755
GCAGAGT70700.043.9266479
GTATCAA81000.041.1011471
AACGCAG76300.041.0068746
TCCAGTA19550.037.1428872
GTACACT21950.032.519376
CCAGTAC22900.032.4903223
AGGACCG2400.032.241235
TAGCGTG10350.032.1902739
ATCCGCG504.3837435E-429.81618160-61
Produced by FastQC (version 0.11.5)