FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25145260
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG540200.21483174164832658No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA492030.1956750496912738No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG430470.17119329845863596No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG418890.16658805675503058No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGC394490.15688443865762375No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA363200.14444074151549835No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC347570.13822485828342998No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA331710.13191750652011552No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC263460.1047752140960165No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG262780.10450478539494124No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG260950.10377701403763571No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA260380.10355033115585205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAGT64700.075.263374
CGACCTA40950.074.2140351
TGATAGT96450.073.3014144
TTCGACG17300.071.963881
GATAGTA112350.071.738355
GACCTAT56800.066.986742
GTACACC226050.063.309647
ATTCAAA648000.063.1286918-19
TATTCAA637350.062.87010616-17
AACCGAT18050.062.214672
CCATTCT100800.062.1634835
GGTACAC209350.061.6385356
CAACGTA14550.061.48991
TTCAAAC653800.061.26082218-19
ACGTATT18650.060.718843
CGTATTC20800.060.712874
GCCTATT95100.060.583933
TTAGTAG131950.060.5689936
TACACCT237100.060.4803668
CTCTGAT180650.060.4581152
Produced by FastQC (version 0.11.5)