FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11284253
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT562290.498296165461728No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG361390.3202604549897986No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG338900.3003300262764403No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC290430.2573763633268414No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT251140.22255793095032522No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC232110.205693722039022No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC183010.1621817589520547No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC162900.14436046409097703No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT160440.1421804349831575No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA135810.120353558184135No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG130240.11541747601724278No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC127810.11326403263025031No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA116600.10332983494786938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA41550.047.319374
GCGACAT42200.046.4771585
CTGCGAC50050.039.570763
CCGACTC155450.038.2180525
CGACTCC158450.037.3445976
ACCCGAC160700.037.061593
CCCGACT169400.035.6601684
GACTCCC168850.035.0444267
ACTCCCT171150.034.93428
CCTCGAC23150.034.9086531
AACCCGA177600.033.619152
GAACCCG180150.033.4859431
GTAGCAA57100.030.939189
GCTGCGA66850.029.5587652
CTCCCTT202800.029.4353569
GGTGTGC92750.028.4171837
CTCGACC28950.027.728892
CGCGTCG14850.026.8630212-13
TTGGACG28750.026.2640237
CGGAACG112050.025.83532156-57
Produced by FastQC (version 0.11.5)