FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11244384
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCNGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT278210.2474212904859884No Hit
AAACCNAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG182200.16203644414847448No Hit
CCCCGNGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG156860.1395007498854539No Hit
CGCCCNATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC143630.12773487636139072No Hit
CTCAGNACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT122730.10914781992503991No Hit
CACGANGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC114910.10219323708617564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT29700.062.7746735
TGCGACA30450.061.526234
CTGCGAC39550.047.6010673
CCTCGAC16400.045.742561
CCGACTC120850.044.806055
CGACTCC123600.043.5926366
ACCCGAC123900.043.4236873
CCCGACT129600.042.059544
AACCCGA137950.039.38982
GAACCCG141550.038.6754761
GCTGCGA54100.035.1293832
GACTCCC166650.034.1216967
ACTCCCT167100.034.112478
CTCGACC23600.031.758172
GCGCGGT25550.029.328046
TTGGACG24300.028.9903967
CTCCCTT196200.028.9825749
GGTGTGC79550.028.0718177
GTAGCAA54600.027.9128599
CGCGTCG12650.027.4805512-13
Produced by FastQC (version 0.11.5)