FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9578416
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG149650.15623668882203487No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA103320.10786752214562408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA59200.044.9160045
TCAACGC59400.044.7542464
ATCAACG61300.043.67483
CGCAGAG62950.042.0706758
ACGCAGA63400.041.7668237
GCAGCGC12700.040.3004538
AACGCAG67100.039.550766
GTATCAA84200.033.8157961
TGCAGCG16500.030.65467
GCAGAGT87100.030.615159
GTCGTAC1403.0705905E-629.7833711
GGTATCA33800.029.4309041
TCTGTCG13400.028.8684678
GCTCTAG23150.027.5319291
GTACGGG93500.025.0371441
TGGTCCT42300.024.188195
GTCTCGC16000.023.4544031
GAGTACG72150.023.43183312-13
AGTACGG86800.022.25457212-13
AATGCGT34350.021.826616
Produced by FastQC (version 0.11.5)