FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11818153
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCNGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT287160.24298213096411933No Hit
AAACCNAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG190410.16111654672265624No Hit
CCCCGNGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG156100.1320849374686552No Hit
CGCCCNATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC147240.12458799611072897No Hit
CTCAGNACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT129270.10938257441750839No Hit
CACGANGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC119750.10132717015933031No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA29700.060.211434
GCGACAT30750.057.867535
CTGCGAC38400.046.5724333
CCTCGAC16600.043.2441521
CCGACTC120100.042.6058125
CGACTCC122800.041.596276
ACCCGAC125450.041.355733
CCCGACT129550.039.839624
AACCCGA136200.038.418492
GAACCCG141000.037.335141
ACTCCCT170700.034.7241068
GACTCCC170650.034.707297
GCTGCGA52500.034.234712
CGGACCA19950.032.3939555
TTGGACG27150.030.7039877
CTCCCTT202500.029.2938799
GGTGTGC86550.028.8415287
GTAGCAA59700.028.2353279
CTCGACC25950.027.9773392
CGCGTCG13300.027.70284812-13
Produced by FastQC (version 0.11.5)