FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13400990
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT810190.6045747366425913No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG437520.326483341902352No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT301050.22464758200700097No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG291790.2177376447560964No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC278830.208066717458934No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC267320.19947779977449429No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC250940.1872548222183585No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC172030.1283711128804663No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA168420.12567728205154993No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT143440.10703686817167986No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA142880.10661898859711111No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG140030.10449228004796661No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC139950.10443258296588535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA54250.050.2601554
GCGACAT55600.049.0966455
CTGCGAC65350.041.94133
CCGACTC217400.040.486385
CGACTCC220050.039.9340976
ACTCCCT226150.039.1298458
ACCCGAC226050.039.0228543
CCCGACT228500.038.916284
GACTCCC227600.038.7136847
GAACCCG229750.038.5484961
AACCCGA235750.037.4379582
CTCCCTT262700.033.6314139
GCTGCGA83650.032.6527672
TTGGACG31500.031.1974127
GTAGCAA72350.030.446569
CCTCGAC24450.030.3153651
GGTGTGC99550.027.6595757
CGGAACG153800.027.45990656-57
TAGTAGC80850.027.2456237
AGTAGCA83750.026.3588818
Produced by FastQC (version 0.11.5)