FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13400990
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358620.26760709469971994No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG309540.23098293484287352No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA248570.18548629616170148No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA182760.13637798401461385No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC166030.1238938317243726No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA160030.1194165505682789No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG156280.11661824984572035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG29400.046.0008853
GTAGCAA420650.045.711179
AGTAGCA428750.044.84268
TAGTAGC438800.042.976197
GGTACGG32650.042.970921
GTACGGT32300.042.1820832
GTACACC82000.040.3187147
GGTGTTA63350.038.0939372
TGATCGT33350.037.8389555
GTAGTAG313550.037.6373836
GCGCGGT29450.036.7040862
GGTAGTA253350.035.839185
GATAGTA66900.035.7719045
GGTACAC81350.034.3175856
TACACCT96250.034.2096378
TGAAGAG322850.033.817028
ACGAGAA392650.033.60229524-25
TGCTAGT46400.033.4812554
AAACGAG396050.033.45607822-23
GCTCTGA216750.033.3519558
Produced by FastQC (version 0.11.5)