FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9552369
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT773860.8101236457678719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361250.37817843929605316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341040.35702138391010646No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG141130.14774345505287745No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127640.1336213037833861No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA96350.1008650314911411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94350.079.606161
GTATCAA295350.070.5277941
ATCAACG367850.056.6103173
TCAACGC372400.055.9982644
CAACGCA375550.055.512725
AACGCAG389600.053.4687546
ACGCAGA422150.049.3157777
CGCAGAG423750.049.157658
TATCAAC445450.046.9624862
GCAGAGT464500.044.8835379
GCAGCGC13900.038.09648
TCTGTCG13000.032.495548
TGCAGCG16500.032.453937
AGAGTAC501700.032.0857510-11
CAGAGTA503450.028.26329210-11
GTACTTT628200.027.38921
AGTACTT525050.027.04957812-13
GCTCTAG22400.026.3036731
GTCTCGC14600.026.0889341
ACGGGAC8950.025.933673
Produced by FastQC (version 0.11.5)