FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004914481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46827053
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC4217060.9005606225102399No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT2602580.555785562674636No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCAT2366710.5054151069468327No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT1858130.39680694832536223No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA1681120.35900614971435424No Hit
CGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAAT1587620.3390390593232506No Hit
CTCTCCTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1422850.3038521343634416No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG1380420.29479113280948943No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA1371930.2929780782916234No Hit
TGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCA1356530.2896893810507358No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG1085610.23183393582337972No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1081140.23087935941644672No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA1053990.22508142889111557No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1034160.2208466973140505No Hit
GGTTAGTTTCTTCTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTCG1004590.21453197150800843No Hit
GTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCT967870.20669035055441137No Hit
CTCTTTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC905420.19335404258730524No Hit
GACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTT835970.17852287223797747No Hit
TCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAA797760.1703630591487361No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC793100.16936790790571424No Hit
GCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCA788220.16832577527353687No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC718470.15343053939354245No Hit
CCGCCGCTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG702290.14997527177292153No Hit
TCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGAC689590.14726316430803366No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG674740.14409192054003483No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG638110.1362695192456378No Hit
TCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG635160.13563954152741578No Hit
CGCCGCTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAAT586950.1253442107492863No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG574820.12275382779266507No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT558420.1192515787828886No Hit
GTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCC555890.11871129280759993No Hit
TGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAAT548720.11718012662466715No Hit
CTCAGAACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT548470.11712673868244496No Hit
CTCAGTACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT542770.11590949359977873No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC533760.11398539216209058No Hit
CGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTTTCCTCCGCTGACTAAT529540.11308420369737981No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG512860.10952216019231448No Hit
GCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGA512470.10943887500244785No Hit
TACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACC504820.10780520397044845No Hit
GCCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCT500890.10696594551871544No Hit
GGTTAGTTTCTTTTCCTCCGCTGACTAATATGCTTAAATTCAGCGGGTCG482980.10314123333791686No Hit
GTGCCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCC482580.10305581263036134No Hit
TTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG480600.1026329801279615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)