FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004914485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44380791
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1838890.41434367404582756No Hit
TCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGT1119540.2522577842292176No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCAT1039230.23416211757018932No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT820870.18496065110691695No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA717120.16158342017833796No Hit
CGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAAT677550.1526674006328549No Hit
CTCTCCTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC616520.1389159557791568No Hit
TGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCA601170.13545725221526583No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG600600.13532881827185098No Hit
CGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCA593600.13375155931763363No Hit
CCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAA474000.106802963471291No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG471230.1061788195708364No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG462290.10416443456359306No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG446070.10050970024396365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)