FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9050592
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT737150.8144771082377815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343390.3794116451166951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326000.36019743238895313No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG134710.14884109238379103No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127110.14044385162871115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91500.082.566971
GTATCAA281750.069.378891
ATCAACG354700.055.125823
TCAACGC358600.054.5594674
CAACGCA361600.054.0550775
AACGCAG372100.052.5477686
ACGCAGA404250.048.3796127
CGCAGAG406650.048.1379478
TATCAAC425500.046.0793762
GCAGAGT441150.044.4002959
GCAGCGC10900.044.188288
TCTGTCG14350.033.5646178
TGCAGCG14650.032.8772857
AGAGTAC470950.032.29158810-11
CAGAGTA472500.028.85697410-11
GCTCTAG24250.027.4745141
AGTACTT493050.027.43111812-13
GTACTTT597200.026.9246441
GTCTCGC14300.026.207661
GTACGGG80750.023.8685591
Produced by FastQC (version 0.11.5)