FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9050592
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG144300.15943708433658263No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA98820.1091862278180256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA58200.041.225225
TCAACGC58650.041.010424
ATCAACG58650.040.9089133
GCAGCGC13550.040.8314978
CGCAGAG59500.039.8948
ACGCAGA59750.039.8257647
AACGCAG64800.036.720846
TCTGTCG14600.034.2277268
GGTATCA35950.032.966451
GTATCAA83800.030.9856381
TGCAGCG18300.029.9074087
GTCTCGC12700.029.0740761
GTACGGG86000.027.2844831
GCAGAGT87050.027.2687599
GCTCTAG22100.024.2531531
GAGTACG65550.023.60550512-13
AATGCGT32850.023.1794016
TGGTCCT42950.022.7332535
AAATGCG33850.022.6888245
CTCGCTC15500.021.8939463
Produced by FastQC (version 0.11.5)