FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9376758
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG151470.16153770844891166No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA119590.12753875060015413No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC112790.12028677715688087No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC107200.11432522839983712No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA99860.10649736294783335No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG98130.10465237558653002No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG94820.10112237086634848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC16950.054.0506138
GCTCTAG18900.052.613661
TGCAGCG18700.048.9670457
CAACGCA46700.048.408425
CGCAGAG47700.047.0189068
ACGCAGA49250.045.7570237
GGTATCA18950.042.1055561
TGGTCCT35250.040.8423275
ATCAACG55700.040.3854873
TCAACGC56150.039.9545634
GCAGAGT61550.036.439679
AACGCAG64600.034.8834886
CATGCAG29700.032.049245
GTATCAA79200.031.0505071
GTCGTGC13200.029.3213021
AAATGCG48050.026.7432735
AATGCGT47800.026.6194526
ACGGGAC7450.026.3600563
GTACACT25350.026.2695876
CCGTATG19100.026.1641779
Produced by FastQC (version 0.11.5)