FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004914504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20761743
Sequences flagged as poor quality0
Sequence length101
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG3065581.4765523299272127No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2871791.38321238250565No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1786620.8605346863218564No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG1686600.8123595403333911No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1620070.7803150246104096No Hit
ACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCG1389020.6690286070875648No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1377400.6634317744902246TruSeq Adapter, Index 2 (100% over 49bp)
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG1180940.5688058078746087No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1156600.5570823220381834No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1014580.4886776606376449No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC893640.4304262893534517No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC844870.406935968719004No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC796570.38367202599511996No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG788810.37993438219517506No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC758090.3651379366366302No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG725640.34950822770515944No Hit
GACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCG704340.33924897346046523No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA684710.3297940832809654No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC657540.3167075134298696No Hit
CCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGC568920.2740232359103954No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG530470.25550359620577134No Hit
GTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCG500400.24102022648098473No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG493240.23757157575835514No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG486790.23446490017721539No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC475760.2291522441059019No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA467470.2251593230876618No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT461170.22212489577585082No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC448140.21584892944681955No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC430810.20750184606369515No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG430210.20721285298637981No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG420050.20231923687717354No Hit
TACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGC386020.1859285128421058No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC383080.18451244676326067No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC377250.18170439736201338No Hit
TACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCC376130.18116494361769145No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC372410.1793731865383364No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC371060.17872295211437692No Hit
ACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCC360230.1735066270688352No Hit
ACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCC353870.17044330044929273No Hit
ACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGG348290.16775566483026016No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG342570.16500059749318735No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG342450.16494279887772428No Hit
CACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGG339520.16353154935016775No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGAC328180.15806958018890804No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA323350.15574318591651962No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG308650.14866285552229405No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG303240.1460571012751675No Hit
ACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCA303220.14604746817259032No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT298520.14378368906695357No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG289940.13965108806134435No Hit
ACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGC286640.13806162613611006No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC283830.1367081752240166No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC282110.1358797284023793No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT274640.13228176458980348No Hit
CAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACG270390.1302347302921532No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT267310.12875123249526788No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC253940.1223115034224246No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT253180.12194544552449187No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG249030.11994657673972749No Hit
ATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGG239980.1155875978235546No Hit
TACCCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCC238520.11488438133542063No Hit
TACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACC237910.11459057170681672No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC233700.11256280361432083No Hit
TGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAG232090.11178733885685802No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG229220.11040498863703302No Hit
CCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGG227800.10972103835405342No Hit
CAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCC227240.10945131148189244No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT225000.10837240399324853No Hit
CGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCC222590.10721161513269864No Hit
TAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAG220470.10619050625951781No Hit
CCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGT208760.1005503247005803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)