FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9397833
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT711000.7565573893470974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324790.34560094864422464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308190.3279373021418874No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG148420.15793002493234343No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131340.13975562238656508No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA113370.12063419301023971No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC112390.11959139942154749No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC109290.11629276664099053No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG106610.1134410453984445No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA105540.1123024850516071No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG104570.11127033221381993No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG103410.11003600510883732No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG102250.10880167800385472No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC98830.10516254119433703No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC96760.1029599057569974No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG95740.1018745491646851No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82250.092.118551
GTATCAA281050.068.868651
GCAGCGC20900.060.9223258
GCTCTAG15050.058.13471
TGCAGCG22300.056.8320057
CAACGCA353750.054.7286155
ATCAACG354450.054.5539473
TCAACGC358650.053.93144
AACGCAG376800.051.4631466
ACGCAGA408300.047.404877
CGCAGAG408400.047.348548
GCAGAGT435450.044.4209339
TATCAAC451000.043.0488972
TGGTCCT33050.039.7967575
CATGCAG33400.039.2015345
TGGTATC24000.031.9913882
GTACTTT614350.030.0234341
AGAGTAC499250.029.75243610-11
TCTGTCG4600.029.7494568
GTGGTAT28050.029.0698171
Produced by FastQC (version 0.11.5)