FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9397833
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG150700.16035611613868858No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA119610.12727402157497372No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC113040.12028304823037397No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC108230.11516484704505817No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA100190.1066096833174201No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG96400.10257683872441659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC15900.060.253958
TGCAGCG18250.052.8148047
CAACGCA47400.046.5570835
GCTCTAG21050.045.275151
CGCAGAG50300.044.362918
ACGCAGA50450.044.1076557
GGTATCA20050.039.5120241
TCAACGC56950.038.7451364
ATCAACG57450.038.419593
GCAGAGT60850.036.5798459
TGGTCCT32750.036.50695
AACGCAG63750.034.903176
CATGCAG30050.032.6610155
GTATCAA77750.031.563771
GTCGTGC12400.029.782561
CCAGTAC23800.027.4969543
TCTGTCG5950.027.0024768
GCCTTTC53850.026.8487517
CTAGCCT75950.026.1550584
TACTAGA55250.025.9822521
Produced by FastQC (version 0.11.5)