FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004914510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48009
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG5961.2414338978108272No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2990.6227998916869754TruSeq Adapter, Index 2 (100% over 49bp)
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2810.5853069216188631No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC2080.4332520985648524No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2080.4332520985648524No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1880.3915932429336166No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1700.3541002728655044No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1480.308275531671145No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1370.2853631610739653No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1280.2666166760399092No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1170.2437043054427295No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1060.2207919348455498No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT940.1957966214668083No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA770.16038659418025789No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG720.14997188027244893No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG710.14788893749088713No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA690.14372305192776355No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC680.14164010914620176No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT640.1333083380199546No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC620.129142452456831No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC620.129142452456831No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG600.12497656689370742No Hit
ACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCG590.12289362411214565No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC550.11456185298589848No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG550.11456185298589848No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC530.1103959674227749No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT530.1103959674227749No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG520.1083130246412131No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG490.10206419629652773No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC490.10206419629652773No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)