FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004914511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48009
Sequences flagged as poor quality0
Sequence length101
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1390.2895290466370889Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTNGGGAAAGAGTGTAGATCTCGGTGGTCGCC1330.27703138994771814Illumina Single End PCR Primer 1 (98% over 50bp)
TGGTGGTAGTAGCAAATATTNAAACGAGAACTTTGAAGGCCGAAGTGGAG770.16038659418025789No Hit
GGGGCGGTACACCTGTCAAANGGTAACGCAGGTGTCCTAAGGCGAGCTCA770.16038659418025789No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG720.14997188027244893No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA720.14997188027244893No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA710.14788893749088713No Hit
GCCCAGTGCTCTGAATGTCANAGTGAAGAAATTCAATGAAGCGCGGGTAA690.14372305192776355No Hit
GATCGTTTTTTCACTGACCCNGTGAGGCGGGGGGGCGAGCCCCGAGGGGC540.11247891020433667No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA530.1103959674227749No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC530.1103959674227749No Hit
ACTCTGATCGTTTTTTCACTNACCCGGTGAGGCGGGGGGGCGAGCCCCGA520.1083130246412131No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGC510.10623008185965133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)