FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8933210
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674710.7552828154717062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311030.3481727173099032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293320.32834781674224606No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG140160.15689768851286381No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133820.1498005756049617No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA107850.12072927872511673No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC104370.1168337025548487No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC103790.11618443985980403No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG99720.11162840681009402No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA99360.11122541617179042No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG96940.10851642354763853No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG96700.1082477631221028No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG96100.10757611205826349No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC93530.10469920666815176No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC90180.10094915489504892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81450.092.593831
GTATCAA270000.068.289161
GCAGCGC20650.057.014748
CAACGCA339800.054.55855
ATCAACG341450.054.1906473
TGCAGCG21900.054.031997
TCAACGC345350.053.613124
GCTCTAG16150.051.1912961
AACGCAG362850.051.0999156
ACGCAGA389200.047.6370967
CGCAGAG390850.047.420788
GCAGAGT417200.044.425729
TATCAAC433000.042.8156742
CATGCAG30900.039.266365
TGGTCCT32050.038.9708825
TGGTATC22050.032.3687932
GTGGTAT24850.031.1150721
GTACTTT587150.030.5618821
AGAGTAC477400.029.6502110-11
GCCTTTC50900.029.4391167
Produced by FastQC (version 0.11.5)