FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8933210
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG141680.15859920454125673No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA113940.12754653702308577No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC108190.12110988099462566No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC104370.1168337025548487No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA93070.10418427418587496No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG92000.10298649645536151No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG89890.10062452354752659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG16050.053.7991141
GCAGCGC15950.053.3337678
CAACGCA46200.049.609745
CGCAGAG48350.047.2456328
ACGCAGA49250.046.2601747
TGCAGCG18400.045.5843777
GGTATCA19450.044.7008061
TCAACGC53450.042.9920164
ATCAACG53900.042.6330833
GCAGAGT59300.038.323259
AACGCAG62600.036.4902236
TGGTCCT33800.036.2826275
GTATCAA75800.032.6818241
CATGCAG27100.032.2920955
GCCTTTC51750.027.1279147
CCAGTAC23850.026.9577453
TCCAGTA22650.026.2833062
CCGTATG20250.026.1468079
CTAGCCT74550.025.6333644
GAGTACG57700.025.47453312-13
Produced by FastQC (version 0.11.5)