FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130411
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80950.25859224236050793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC10700.044.8326878
TCCGACC11350.043.102119
TTTCCGA14600.034.1591877
GTTTCCG12500.033.0582856
TCCGTTT14850.032.640213
ATAGCGC15550.032.22295894-95
CGTTTCC12750.032.0513765
CCGATCG17650.031.48360486-87
ACCCGAT17850.031.39692384-85
CTCCGTT11600.030.7243352
GGACCGT2650.030.485211
CCGTTTC14500.030.478544
CGGCATA16450.030.45999790-91
GATCGGC18600.029.74789888-89
CATAGCG18850.029.35336394-95
TCGGCAT19500.028.49669690-91
ATCGGCA17950.028.31148188-89
GCTCCGT10500.028.0600591
TGTCGGG5500.027.646855
CCCGATC18800.027.53670584-85
Produced by FastQC (version 0.11.5)