FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12603616
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1098890.8718847035644375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488150.3873094832467127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425720.3377760794997245No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG189790.1505837689755067No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131740.1045255583794365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151600.084.767951
GTATCAA461800.072.037471
ATCAACG578850.056.986033
CAACGCA580250.056.9711725
TCAACGC581250.056.750734
AACGCAG605900.054.600336
ACGCAGA657950.050.188717
CGCAGAG667700.049.473468
TATCAAC671250.049.293612
GCAGAGT715350.046.1779989
GCTCTAG27700.038.6786231
GCAGCGC32250.035.4222158
TGCAGCG33250.034.1780787
AGAGTAC770350.031.40389310-11
CAGAGTA752700.030.49216310-11
GAGTACG161950.026.43346212-13
AGTACTT756700.026.05751212-13
TGGTCCT69900.025.4600035
AGTACGG187800.024.52164312-13
GAGTACT764450.024.46242312-13
Produced by FastQC (version 0.11.5)