FastQCFastQC Report
Fri 24 Mar 2023
EGAF00004914548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63839479
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG8959971.4035155268106119No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2716200.42547339711215376No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2627890.4116402641694491No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC2590520.4057865196550241No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG2211300.3463844058000536No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC2200710.3447255576756822No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA2139420.33512491541480155No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1766310.2766798895711539No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1560990.24451797296152744No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1007980.15789289257827432No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC965600.15125436722314103No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT911100.1427173301335996No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC892810.139852331814926No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT883450.13838615443587815No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT873760.13686828490564593No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA816610.1279161441778057No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT791260.12394524710955114No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG791110.12392175067719459No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT751760.11775785325566332No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA725800.11369140402915881No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG696010.10902501256315077No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG695010.10886836968077387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)