FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17625468
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA270250.1533292619520798No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA215920.12250454853170423No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG205940.116842287535287No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196170.11129917231133947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG5500.040.483681
TAGTAGC221100.037.4382067
TGATCGT40150.036.222875
AGTAGCA231550.036.2224668
CAGTACG116100.035.8967178
GTAGCAA234050.035.859059
CGGTACA69100.035.5125435
GTACACC88550.035.188417
TACCGAC10850.034.4813586
CACCGTC59600.033.218029
GGCGGTA79350.033.016083
TCGACGT8350.032.907042
TCGATCG73400.032.2597476
CGAGAAC171050.031.63384424-25
CGTCGGT37200.031.3210189
CGCGACA34750.031.23583260-61
AAACGAG177950.031.13913522-23
TCAGTCG160500.031.00260584-85
AAGGCCG176950.030.99711636-37
CGAAGTG169050.030.92903940-41
Produced by FastQC (version 0.11.5)