FastQCFastQC Report
Thu 12 May 2022
EGAF00004914560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36804514
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG5599251.5213487128236498No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1852850.5034300955583871No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1795010.4877146319606339No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1474330.4005840153194252No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1464470.39790499610998803No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1390750.37787484437370916No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1354080.36791139260798283No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1178480.3201998537462008No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG791670.21510133240721505No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC777110.21114529592755932No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC720400.19573685988626285No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT707300.1921775138777814No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT689050.18721888298810302No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT648260.17613600331741916No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA598270.16255343026673305No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC557760.15154662822065793No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT556360.15116624009761412No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG542380.1473677929832194No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA520620.14145547472790973No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG516790.14041484150558273No Hit
CGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTC498700.1354996835442522No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC494320.1343096121307294No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT477630.1297748422924427No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG471640.1281473245374195No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG460920.12523463833811255No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT454910.12360168646704586No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCG451670.1227213596680016No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC434640.11809420985697569No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC419660.11402405694040682No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG408930.11110865368307811No Hit
CCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGT406740.1105136179763167No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT371380.10090610081143851No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT370890.10077296496837317No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)